Boncinelli E, Goyns M H, Scotto L, Simeone A, Harris P, Kellow J E, Young B D
Mol Biol Med. 1984 Feb;2(1):1-14.
Human metaphase chromosomes were fractionated by a fluorescent-activated cell sorter (FACS II) and the chromosome 6 fraction was sorted. A genomic library was constructed in lambda gtWES cloning arms using a partial EcoRI digest of the chromosomal DNA. We estimate that at least 60% of our library represents chromosome 6 material, and as 1.2 x 10(5) recombinants were obtained, this indicates that the majority of clonable chromosome 6 sequences are represented. The library was screened with a mouse H-2 class I clone and 18 HLA class I recombinants were isolated from 4 x 10(4) plaques.
用人荧光激活细胞分选仪（FACS II）对人中期染色体进行分离，并分选6号染色体部分。使用染色体DNA的部分EcoRI酶切片段，在λgtWES克隆臂中构建基因组文库。我们估计，我们的文库中至少60%代表6号染色体物质，由于获得了1.2×10⁵个重组体，这表明大多数可克隆的6号染色体序列都有代表。用小鼠H-2 I类克隆筛选该文库，从4×10⁴个噬菌斑中分离出18个HLA I类重组体。
