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在48S起始前复合物中，信使核糖核酸（mRNA）与起始因子eIF-3、24 kDa帽结合蛋白以及核糖体蛋白S1、S3/3a、S6和S11发生交联。

Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.

作者信息

Westermann P, Nygård O

出版信息

Nucleic Acids Res. 1984 Dec 11;12(23):8887-97. doi: 10.1093/nar/12.23.8887.

DOI:10.1093/nar/12.23.8887
PMID:6514574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320426/
Abstract

Native small ribosomal subunits from rabbit reticulocytes contain all initiation factors necessary for the formation of the mRNA-containing 48S pre-initiation complex. The complex formed in the presence of Met-tRNAf and 125I-labelled globin mRNA was cross-linked with diepoxybutane, and the covalent mRNA-protein complexes were isolated under denaturating conditions. The proteins of the covalent complex were identified as the 110, 95 and 66/64 kDa subunits of eIF-3. In addition, the 24 kDa cap binding protein and the ribosomal proteins S1, S3/3a, S6 and S11 were found covalently linked to the mRNA. Ribosomal proteins S3/3a and S6 were also involved in the ribosomal mRNA-binding domain of reticulocyte polysomes.

摘要

来自兔网织红细胞的天然小核糖体亚基包含形成含mRNA的48S前起始复合物所需的所有起始因子。在甲硫氨酰 - tRNAf和125I标记的珠蛋白mRNA存在下形成的复合物用双环氧丁烷交联，并且在变性条件下分离共价mRNA - 蛋白质复合物。共价复合物的蛋白质被鉴定为eIF - 3的110、95和66/64 kDa亚基。此外，发现24 kDa帽结合蛋白和核糖体蛋白S1、S3/3a、S6和S11与mRNA共价连接。核糖体蛋白S3/3a和S6也参与网织红细胞多核糖体的核糖体mRNA结合结构域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/0738ee9eec9b/nar00341-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/8e766b2ef565/nar00341-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/7a2ae1da9707/nar00341-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/7373f13bdd43/nar00341-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/0738ee9eec9b/nar00341-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/8e766b2ef565/nar00341-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/7a2ae1da9707/nar00341-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/7373f13bdd43/nar00341-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/834d/320426/0738ee9eec9b/nar00341-0156-a.jpg

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Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.在48S起始前复合物中，信使核糖核酸（mRNA）与起始因子eIF-3、24 kDa帽结合蛋白以及核糖体蛋白S1、S3/3a、S6和S11发生交联。
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本文引用的文献

1
Spatial arrangement of proteins within the small subunit of rat liver ribosomes studied by cross-linking.通过交联研究大鼠肝脏核糖体小亚基内蛋白质的空间排列。
EMBO J. 1983;2(2):255-60. doi: 10.1002/j.1460-2075.1983.tb01414.x.
2
Proteins crosslinked to messenger RNA by irradiating polyribosomes with ultraviolet light.通过用紫外线照射多核糖体与信使核糖核酸交联的蛋白质。
Nucleic Acids Res. 1980 Dec 11;8(23):5685-701. doi: 10.1093/nar/8.23.5685.
3
Cross-linking of mRNA to proteins by irradiation of intact cells with ultraviolet light.
真核生物核糖体蛋白在 5.5Å 冷冻电镜真核 80S 核糖体图谱中的定位。
Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19754-9. doi: 10.1073/pnas.1010005107. Epub 2010 Oct 25.
4
Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.真核生物翻译起始复合物中核糖体的位置及与mRNA的接触
EMBO J. 2008 Jun 4;27(11):1609-21. doi: 10.1038/emboj.2008.90. Epub 2008 May 8.
5
Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast.PRT1 中的 RNP1 基序与 HCR1 的相互作用促进了酵母中真核起始因子 3 与 40S 核糖体亚基的结合。
Mol Cell Biol. 2006 Apr;26(8):2984-98. doi: 10.1128/MCB.26.8.2984-2998.2006.
6
Eukaryotic translation initiation factor 3 (eIF3) and eIF2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast.在酵母中，真核生物翻译起始因子3（eIF3）和eIF2可独立于eIF4G促进mRNA与40S亚基结合。
Mol Cell Biol. 2006 Feb;26(4):1355-72. doi: 10.1128/MCB.26.4.1355-1372.2006.
7
Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association.真核生物起始因子3与核糖体40S亚基的结合及其在核糖体解离和抗缔合中的作用。
RNA. 2005 Apr;11(4):470-86. doi: 10.1261/rna.7215305. Epub 2005 Feb 9.
8
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9
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Virus Genes. 1999;19(2):153-61. doi: 10.1023/a:1008131325056.
10
Specific interaction of eukaryotic translation initiation factor 3 with the 5' nontranslated regions of hepatitis C virus and classical swine fever virus RNAs.真核生物翻译起始因子3与丙型肝炎病毒和经典猪瘟病毒RNA的5'非翻译区的特异性相互作用。
J Virol. 1998 Jun;72(6):4775-82. doi: 10.1128/JVI.72.6.4775-4782.1998.
通过用紫外线照射完整细胞使信使核糖核酸与蛋白质交联。
Eur J Biochem. 1980 Nov;112(2):323-30. doi: 10.1111/j.1432-1033.1980.tb07207.x.
4
Proximity of mRNA5'-region and 18S rRNA in eukaryotic initiation complexes.真核生物起始复合物中mRNA 5′区域与18S rRNA的接近度。
Nature. 1980 Jul 17;286(5770):226-30. doi: 10.1038/286226a0.
5
The mechanism of action of eukaryotic initiation factor 4C in protein synthesis.真核生物起始因子4C在蛋白质合成中的作用机制。
Eur J Biochem. 1980;107(1):39-45. doi: 10.1111/j.1432-1033.1980.tb04621.x.
6
Interaction of synthetic polynucleotides with small rat liver ribosomal subunits possessing low and highly phosphorylated protein S6.合成多核苷酸与具有低磷酸化和高磷酸化蛋白S6的大鼠肝脏小核糖体亚基的相互作用。
Biochim Biophys Acta. 1980 Jul 29;608(2):459-68. doi: 10.1016/0005-2787(80)90191-4.
7
Ribosomal proteins cross-linked to natural mRNA by UV irradiation of rat liver polysomes.通过紫外线照射大鼠肝脏多核糖体使核糖体蛋白与天然信使核糖核酸交联。
J Biochem. 1981 Nov;90(5):1549-52. doi: 10.1093/oxfordjournals.jbchem.a133624.
8
Translation in vivo and in vitro of mRNAs coding for vitellogenin, serum albumin and very-low-density lipoprotein II from chicken liver. A difference in translational efficiency.鸡肝中编码卵黄蛋白原、血清白蛋白和极低密度脂蛋白II的mRNA的体内和体外翻译。翻译效率的差异。
Eur J Biochem. 1981 Mar;114(3):635-41. doi: 10.1111/j.1432-1033.1981.tb05191.x.
9
ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.ATP/镁离子依赖的帽结合蛋白与真核生物mRNA 5'端的交联。
Nucleic Acids Res. 1981 Apr 10;9(7):1643-56. doi: 10.1093/nar/9.7.1643.
10
Purification of protein synthesis initiation factor eIF-3 from rat liver microsomes by affinity chromatography on rRNA-cellulose.通过rRNA-纤维素亲和层析从大鼠肝脏微粒体中纯化蛋白质合成起始因子eIF-3。
Biochim Biophys Acta. 1982 Jun 30;697(3):263-9. doi: 10.1016/0167-4781(82)90088-4.