Miltenburger H G, Naser W L, Harvey J P, Huber J, Huger A M
Z Naturforsch C Biosci. 1984 Sep-Oct;39(9-10):993-1002. doi: 10.1515/znc-1984-9-1021.
We established more than 200 primary cell lines of Cydia pomonella (coding moth). 81 of them were selected and screened for replication of two baculoviruses (from two different subgroups): the Choristoneura murinana NPV and the Cydia pomonella GV. Although all these cell lines had been derived from the same insect species, they varied largely in their response to challenge with the NPV. Most of them showed CPE or produced different amounts of polyhedra. Interestingly, we also found a few cell lines that were permissive for GV replication. To our knowledge this is the first time that GV replication in cell lines has been obtained. Our results show that cell line properties are most important for baculovirus in vitro replication.
我们建立了200多个苹果蠹蛾（编码蛾）的原代细胞系。从中挑选了81个并针对两种杆状病毒（来自两个不同亚组）进行复制筛选：鼠毒蛾核型多角体病毒（Choristoneura murinana NPV）和苹果蠹蛾颗粒体病毒（Cydia pomonella GV）。尽管所有这些细胞系都源自同一昆虫物种，但它们对NPV攻击的反应差异很大。大多数细胞系表现出细胞病变效应（CPE）或产生了不同数量的多角体。有趣的是，我们还发现了一些对GV复制具有容许性的细胞系。据我们所知，这是首次在细胞系中实现GV复制。我们的结果表明，细胞系特性对于杆状病毒的体外复制最为重要。
