Romanenko V I, Aurora Pubienes M, Daukshta A S
Mikrobiologiia. 1978 Jan-Feb;47(1):149-57.
The formation and number of bacteria in the surface film of water depend on the content of organic substances in water. In flasks containing distilled water after 1-2 days, a bacterial film 5-7 mcm thick is formed at the surface; in flasks with water taken from reservoirs, the film is 5-15 mcm thick; in flasks with water containing organic substances, the film is 15-50 mcm thick. A distinct bacterial band of growth is formed on glasses semisubmerged in water in this layer. At the beginning, bacterial rodshaped cells are oriented by their side surface to the surface layer of water. Judging by the assimilation of protein labeled hydrolysate, the total activity of bacterial population in a film is much higher than in deep water layers. In the course of time, the number of bacteria changes, they begin to die off, and their species are substituted by other bacterial species. Numerous motile forms and various Caulobacterial forms are found in the surface layer of water. The dimensions of bacterial cells growing on glass are much larger than in dry preparations obtained from this film on membrane filters.
水表面膜中细菌的形成和数量取决于水中有机物质的含量。在装有蒸馏水的烧瓶中，1 - 2天后，水面会形成一层5 - 7微米厚的细菌膜；在装有取自水库水的烧瓶中，该膜厚5 - 15微米；在装有含有有机物质水的烧瓶中，膜厚15 - 50微米。在这一层半浸于水中的玻璃上会形成一条明显的细菌生长带。起初，杆状细菌细胞以其侧面朝向水的表层。从标记蛋白质水解产物的同化情况判断，膜中细菌群体的总活性远高于深水层。随着时间推移，细菌数量发生变化，它们开始死亡，其种类被其他细菌种类所取代。在水的表层发现大量游动形态和各种柄杆菌形态。在玻璃上生长的细菌细胞尺寸比从该膜在膜滤器上获得的干燥制剂中的细菌细胞尺寸大得多。
