Pajari M
Acta Physiol Scand. 1984 Nov;122(3):415-20. doi: 10.1111/j.1748-1716.1984.tb07527.x.
The accumulation of labelled leucine, isoleucine and valine by cerebral slices of developing and adult rats was studied. The accumulation increased with age by 15-25%. It was strongly (from 52 to 86%) inhibited by a 100-fold excess of phenylalanine, tryptophan and another branched-chain amino acid, but moderately activated by GABA and glutamate. The inhibitions evoked by leucine and isoleucine were slightly stronger in young than in adult rats. The corresponding 2-oxoacid analogs of leucine, isoleucine and valine were also inhibitory but less effective. The 30-min accumulation of 3H-labelled branched-chain amino acids was ostensibly higher than the increase in their total concentrations in incubated slices, which apparently bespeaks lively homoexchange of endogenous intracellular and labelled extracellular amino acids.
研究了发育中和成年大鼠脑切片对标记亮氨酸、异亮氨酸和缬氨酸的积累情况。积累量随年龄增长增加了15% - 25%。它受到过量100倍的苯丙氨酸、色氨酸和另一种支链氨基酸的强烈抑制（从52%到86%），但受到γ-氨基丁酸和谷氨酸的适度激活。亮氨酸和异亮氨酸引起的抑制在幼鼠中比成年大鼠略强。亮氨酸、异亮氨酸和缬氨酸相应的2-氧代酸类似物也具有抑制作用，但效果较差。3H标记的支链氨基酸30分钟的积累量表面上高于孵育切片中其总浓度的增加，这显然表明内源性细胞内氨基酸和标记的细胞外氨基酸之间存在活跃的同源交换。
