Van Blerkom J, Runner M N
Am J Anat. 1984 Nov;171(3):335-55. doi: 10.1002/aja.1001710309.
Correlated nuclear and cytoplasmic reorganizations during the 14 hr of reactivated meiosis in vivo and in vitro were examined in the laboratory mouse. Observations of living oocytes by differential interference contrast microscopy, and by fluorescent microscopy with nontoxic mitochondrial and DNA-specific probes, enabled us to determine that the major cytoplasmic reorganization involved two mitochondrial translocations associated with two stages of nuclear maturation. These observations were confirmed at the fine structural level by parallel transmission electron microscopy. Mitochondria translocate to the perinuclear region during formation of the first metaphase spindle and subsequently disperse during abstriction of the first polar body. Determinations of frequency of maturation in more than 2,900 normal oocytes, and in more than 1,100 oocytes in which germinal vesicle breakdown was reversibly inhibited, indicated that mitochondrial redistributions are a normal and probably necessary feature of reactivated meiosis in the laboratory mouse. We suggest that these two rapid translocations serve to concentrate mitochondria for localized activities that require elevated levels of adenosine triphosphate.
我们在实验室小鼠体内和体外对重新激活减数分裂的14小时内相关的细胞核和细胞质重组进行了研究。通过微分干涉相差显微镜观察活卵母细胞，并使用无毒的线粒体和DNA特异性探针进行荧光显微镜观察，使我们能够确定主要的细胞质重组涉及与核成熟两个阶段相关的两次线粒体易位。这些观察结果在精细结构水平上通过平行透射电子显微镜得到了证实。线粒体在第一次中期纺锤体形成期间易位到核周区域，随后在第一次极体缢缩期间分散。对2900多个正常卵母细胞以及1100多个生发泡破裂被可逆抑制的卵母细胞的成熟频率测定表明，线粒体重新分布是实验室小鼠重新激活减数分裂的正常且可能必要的特征。我们认为这两次快速易位有助于将线粒体集中用于需要升高三磷酸腺苷水平的局部活动。
