Robins S P, Reeds P J
Anal Biochem. 1984 Oct;142(1):24-7. doi: 10.1016/0003-2697(84)90511-6.
A method for measuring keto acid concentrations in physiological fluids using an amino acid analyzer was developed. After preliminary deproteinization and removal of amino acids, reduction with sodium cyanoborohydride at 105 degrees C resulted in efficient conversion of the keto acids to their corresponding amino acids. In applying the technique to plasma samples, the use of MeOH for deproteinization was necessary to avoid the large losses of keto acids that occurred during precipitation of proteins with perchloric acid. The method was used to follow plasma ketoisocaproate concentrations in rat plasma after administration of leucine, and was sufficiently sensitive to detect concomitant changes in other branched-chain keto acid concentrations.
开发了一种使用氨基酸分析仪测量生理流体中酮酸浓度的方法。经过初步的脱蛋白和去除氨基酸后，在105℃下用氰基硼氢化钠还原可使酮酸有效地转化为相应的氨基酸。在将该技术应用于血浆样本时，必须使用甲醇进行脱蛋白，以避免在用高氯酸沉淀蛋白质过程中发生的酮酸大量损失。该方法用于跟踪大鼠血浆中亮氨酸给药后血浆酮异己酸的浓度，并且灵敏度足以检测其他支链酮酸浓度的伴随变化。
