Töpfer-Petersen E, Januschke E, Schmoeckel C, Schill W B
Andrologia. 1984 Nov-Dec;16(6):538-47.
The distribution of the main lectin receptors of boar spermatozoa were analyzed by concanavalin A and Ricinus communis agglutinin-120 visualized for light microscopy by enzymatic amplification with 3-amino-9-ethylcarbazole. The lectin binding sites of the acrosomal membranes in addition to the plasma membrane were displayed by electron microscopy using the lectin-peroxidase and lectin-ferritin technique. Lectin binding was particularly prominent at the anterior part of the head. It could be shown that both acrosomal membranes contain receptor sites for concanavalin A and Ricinus communis-agglutinin-120. Additionally, the whole cell surface could be labeled with both lectins.
通过伴刀豆球蛋白A和蓖麻凝集素-120对猪精子主要凝集素受体进行分布分析，利用3-氨基-9-乙基咔唑酶促扩增进行光学显微镜观察。采用凝集素-过氧化物酶和凝集素-铁蛋白技术，通过电子显微镜显示除质膜外顶体膜的凝集素结合位点。凝集素结合在头部前端尤为突出。结果表明，两种顶体膜均含有伴刀豆球蛋白A和蓖麻凝集素-120的受体位点。此外，两种凝集素均可标记整个细胞表面。
