Viola-Magni M P, Biagetti M
Basic Appl Histochem. 1984;28(3):257-64.
DNA synthesis in newborn rat hepatocytes was studied in the first three days of life by means of repeated injections of (3H) thymidine. One group of animals was treated with the label adsorbed on activated charcoal (experimental group) and another group (controls) was given the label diluted in saline. The specific activity of DNA was higher in control group, but its increase was not linear with time; in the experimental group, the radioactivity was lower, but its increase with time was linear. The percentage of labeled nuclei was higher in the experimental animals than in the controls and increased linearly with time. The average number of grains/nucleus was considerably smaller in the experimental group than in the controls, in which also the percentage of labeled cells showed considerable variations during the first three days of life. It is concluded that activated charcoal adsorption increases label availability with time and, by keeping a lower label concentration in the pool, reduces the risk of radiation damage.
通过重复注射（3H）胸腺嘧啶核苷，研究新生大鼠肝细胞在出生后前三天的DNA合成情况。一组动物接受吸附在活性炭上的标记物处理（实验组），另一组（对照组）给予稀释在盐水中的标记物。对照组中DNA的比活性较高，但其增加与时间不成线性关系；在实验组中，放射性较低，但其随时间的增加呈线性。实验组动物中标记细胞核的百分比高于对照组，且随时间呈线性增加。实验组中每个细胞核的平均颗粒数明显少于对照组，在对照组中，标记细胞的百分比在出生后的前三天也有相当大的变化。结论是，活性炭吸附随时间增加标记物的可用性，并通过在池中保持较低的标记物浓度，降低辐射损伤的风险。
