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1
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Biochem J. 1984 Dec 1;224(2):637-43. doi: 10.1042/bj2240637.
2
Comparison of glycerolipid biosynthesis in non-green plastids from sycamore (Acer pseudoplatanus) cells and cauliflower (Brassica oleracea) buds.悬铃木（Acer pseudoplatanus）细胞和花椰菜（Brassica oleracea）芽中非绿色质体甘油脂质生物合成的比较。
Biochem J. 1989 May 1;259(3):775-83. doi: 10.1042/bj2590775.
3
Site of synthesis of phosphatidic acid and diacyglycerol in spinach chloroplasts.菠菜叶绿体中磷脂酸和二酰甘油的合成位点。
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The molecular species of phosphatidic acid, diacylglycerol and phosphatidylcholine synthesized from sn-glycerol 3-phosphate in rat lung microsomes.大鼠肺微粒体中由sn-甘油-3-磷酸合成的磷脂酸、二酰基甘油和磷脂酰胆碱的分子种类。
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[Direction of fatty acid and glycerolipid biosynthesis in isolated chloroplasts from developing pea leaves].
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1
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Planta. 1991 Feb;183(3):353-8. doi: 10.1007/BF00197733.
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3
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Phosphatidate phosphatases of mammals, yeast, and higher plants.哺乳动物、酵母和高等植物的磷脂酸磷酸酶。
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6
Comparison of glycerolipid biosynthesis in non-green plastids from sycamore (Acer pseudoplatanus) cells and cauliflower (Brassica oleracea) buds.悬铃木（Acer pseudoplatanus）细胞和花椰菜（Brassica oleracea）芽中非绿色质体甘油脂质生物合成的比较。
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本文引用的文献

1
Rates and products of long-chain Fatty Acid synthesis from [1-C]acetate in chloroplasts isolated from leaves of 16:3 and 18:3 plants.从16:3和18:3植物叶片中分离出的叶绿体中，由[1-C]乙酸盐合成长链脂肪酸的速率和产物。
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2
Similarities and differences in lipid metabolism of chloroplasts isolated from 18:3 and 16:3 plants.从 18:3 和 16:3 植物中分离的叶绿体在脂质代谢方面的相似性和差异。
Plant Physiol. 1983 Jun;72(2):273-9. doi: 10.1104/pp.72.2.273.
3
Phosphatidylglycerol synthesis in spinach chloroplasts: characterization of the newly synthesized molecule.菠菜叶绿体中磷脂酰甘油的合成：新合成分子的特性。
Plant Physiol. 1982 Nov;70(5):1260-4. doi: 10.1104/pp.70.5.1260.
4
Positional Specificity and Fatty Acid Selectivity of Purified sn-Glycerol 3-Phosphate Acyltransferases from Chloroplasts.叶绿体中纯化的 sn-甘油-3-磷酸酰基转移酶的位置特异性和脂肪酸选择性。
Plant Physiol. 1981 Sep;68(3):653-7. doi: 10.1104/pp.68.3.653.
5
Enzymic determination of metabolites in the subcellular compartments of spinach protoplasts.酶法测定菠菜原生质体亚细胞区室代谢物。
Plant Physiol. 1980 Jul;66(1):187-93. doi: 10.1104/pp.66.1.187.
6
Phosphatidic Acid synthesis in castor bean endosperm.菜豆胚乳中磷脂酸的合成。
Plant Physiol. 1977 Mar;59(3):459-63. doi: 10.1104/pp.59.3.459.
7
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
8
The role of chloroplasts and microsomal fractions in polar-lipid synthesis from [1-14C]acetate by cell-free preparations from spinach (Spinacia oleracea) leaves.叶绿体和微粒体部分在菠菜（Spinacia oleracea）叶片无细胞制剂利用[1-14C]乙酸合成极性脂质中的作用。
Biochem J. 1980 Apr 15;188(1):17-24. doi: 10.1042/bj1880017.
9
Synthesis of phosphatidylglycerol by chloroplasts from leaves of Spinacia oleracea L. (spinach).菠菜（Spinacia oleracea L.）叶片叶绿体合成磷脂酰甘油。
Arch Biochem Biophys. 1981 Jul;209(2):584-91. doi: 10.1016/0003-9861(81)90316-7.
10
Relationship between fatty-acyl composition of diacylgalactosylglycerol and turnover of chloroplast phosphatidate.二酰基半乳糖基甘油的脂肪酰组成与叶绿体磷脂酸周转之间的关系。
Biochem J. 1983 Mar 15;210(3):949-52. doi: 10.1042/bj2100949.

分离的完整叶绿体中的甘油脂标记动力学

Glycerolipid labelling kinetics in isolated intact chloroplasts.

作者信息

Gardiner S E, Roughan P G, Browse J

出版信息

Biochem J. 1984 Dec 1;224(2):637-43. doi: 10.1042/bj2240637.

DOI:10.1042/bj2240637
PMID:6517868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144475/
Abstract

Glycerolipid synthesis was studied in intact chloroplasts isolated from three different plant species. The sequential acylation of sn-glycerol 3-phosphate and lysophosphatidate (1-acyl-sn-glycerol 3-phosphate) was confirmed by monitoring the incorporation of oleate synthesized in situ into lysophosphatidate, phosphatidate and diacylglycerol. Lysophosphatidate was not only readily detected in these experiments, but was also present in the chloroplasts at the beginning of the time courses. The rate of glycerolipid synthesis depended primarily on sn-glycerol 3-phosphate supply, and given adequate sn-glycerol 3-phosphate, the proportion of newly synthesized fatty acids diverted into glycerolipids appeared to be determined by differing acyltransferase activities in the chloroplasts isolated from different plant species.

摘要

对从三种不同植物物种中分离出的完整叶绿体中的甘油脂质合成进行了研究。通过监测原位合成的油酸掺入溶血磷脂酸、磷脂酸和二酰基甘油中的情况，证实了sn-甘油-3-磷酸和溶血磷脂酸（1-酰基-sn-甘油-3-磷酸）的顺序酰化。在这些实验中不仅很容易检测到溶血磷脂酸，而且在时间进程开始时叶绿体中就已存在。甘油脂质合成的速率主要取决于sn-甘油-3-磷酸的供应，并且在有足够的sn-甘油-3-磷酸的情况下，新合成的脂肪酸转入甘油脂质中的比例似乎由从不同植物物种中分离出的叶绿体中不同的酰基转移酶活性决定。