分离的完整叶绿体中的甘油脂标记动力学
Glycerolipid labelling kinetics in isolated intact chloroplasts.
作者信息
Gardiner S E, Roughan P G, Browse J
出版信息
Biochem J. 1984 Dec 1;224(2):637-43. doi: 10.1042/bj2240637.
Abstract
Glycerolipid synthesis was studied in intact chloroplasts isolated from three different plant species. The sequential acylation of sn-glycerol 3-phosphate and lysophosphatidate (1-acyl-sn-glycerol 3-phosphate) was confirmed by monitoring the incorporation of oleate synthesized in situ into lysophosphatidate, phosphatidate and diacylglycerol. Lysophosphatidate was not only readily detected in these experiments, but was also present in the chloroplasts at the beginning of the time courses. The rate of glycerolipid synthesis depended primarily on sn-glycerol 3-phosphate supply, and given adequate sn-glycerol 3-phosphate, the proportion of newly synthesized fatty acids diverted into glycerolipids appeared to be determined by differing acyltransferase activities in the chloroplasts isolated from different plant species.
摘要
对从三种不同植物物种中分离出的完整叶绿体中的甘油脂质合成进行了研究。通过监测原位合成的油酸掺入溶血磷脂酸、磷脂酸和二酰基甘油中的情况，证实了sn-甘油-3-磷酸和溶血磷脂酸（1-酰基-sn-甘油-3-磷酸）的顺序酰化。在这些实验中不仅很容易检测到溶血磷脂酸，而且在时间进程开始时叶绿体中就已存在。甘油脂质合成的速率主要取决于sn-甘油-3-磷酸的供应，并且在有足够的sn-甘油-3-磷酸的情况下，新合成的脂肪酸转入甘油脂质中的比例似乎由从不同植物物种中分离出的叶绿体中不同的酰基转移酶活性决定。
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