分离的叶绿体对外源脂质中亚油酸的去饱和作用。
Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts.
作者信息
Jones A V, Harwood J L
出版信息
Biochem J. 1980 Sep 15;190(3):851-4. doi: 10.1042/bj1900851.
Abstract
When [14C]diacylgalactosylglycerol was added to isolated pea or lettuce chloroplasts linolenate synthesis was seen. The desaturation of [14C]linoleate in diacylgalactosylglycerol to [14C]linolenate was stimulated by the addition of a soluble protein fraction containing lipid-exchange activity. Other [14C]acyl lipids were ineffective, except that [14C]phosphatidylcholine in the presence of UDP-galactose and sn-glycerol 3-phosphate could also supply [14C]linoleate for desaturation. These results are consistent with a role of diacylgalactosylglycerol in linolenate synthesis, as indirectly suggested by labelling experiments.
摘要
当向分离的豌豆或生菜叶绿体中添加[14C]二酰基半乳糖基甘油时，可观察到亚麻酸的合成。含有脂质交换活性的可溶性蛋白质组分的添加刺激了二酰基半乳糖基甘油中[14C]亚油酸去饱和生成[14C]亚麻酸。其他[14C]酰基脂质无效，不过在UDP-半乳糖和sn-甘油3-磷酸存在下的[14C]磷脂酰胆碱也可以提供[14C]亚油酸用于去饱和。这些结果与标记实验间接表明的二酰基半乳糖基甘油在亚麻酸合成中的作用一致。
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