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1
Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts.分离的叶绿体对外源脂质中亚油酸的去饱和作用。
Biochem J. 1980 Sep 15;190(3):851-4. doi: 10.1042/bj1900851.
2
Linoleate and alpha-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts.菠菜（Spinacia oleracea）离体叶绿体合成亚油酸和α-亚麻酸
Biochem J. 1979 Dec 15;184(3):571-4. doi: 10.1042/bj1840571.
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The incorporation of [14C]acetate into the constituent fatty acids of monogalactosyldiglyceride by isolated spinach chloroplasts.分离的菠菜叶绿体将[14C]乙酸盐掺入单半乳糖二酰甘油的组成脂肪酸中。
Arch Biochem Biophys. 1979 Oct 1;197(1):322-32. doi: 10.1016/0003-9861(79)90252-2.
4
Fatty acid biosynthesis in the leaves of barley, wheat and pea.大麦、小麦和豌豆叶片中的脂肪酸生物合成。
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Relationship between fatty-acyl composition of diacylgalactosylglycerol and turnover of chloroplast phosphatidate.二酰基半乳糖基甘油的脂肪酰组成与叶绿体磷脂酸周转之间的关系。
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Biosynthesis of gamma-linolenic acid in cotyledons and microsomal preparations of the developing seeds of common borage (Borago officinalis).普通琉璃苣（Borago officinalis）发育种子子叶和微粒体制剂中γ-亚麻酸的生物合成
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Biochem J. 1978 Feb 15;170(2):421-33. doi: 10.1042/bj1700421.

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Direct desaturation of intact galactolipids by a desaturase solubilized from spinach (Spinacia oleracea) chloroplast envelopes.菠菜（Spinacia oleracea）叶绿体被膜中溶解的去饱和酶对完整半乳糖脂的直接去饱和作用。
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The thylakoid membranes of higher plant chloroplasts.高等植物叶绿体的类囊体膜。
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本文引用的文献

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Fatty acid metabolism in the chloroplast lipids of green and blue-green algae.绿藻和蓝藻叶绿体脂质中的脂肪酸代谢
Lipids. 1968 Jul;3(4):354-60. doi: 10.1007/BF02530939.
2
Elongation Pathway for alpha-Linolenic Acid Synthesis in Spinach Leaves: A Reexamination.菠菜叶片中α-亚麻酸合成的延伸途径：再探讨。
Plant Physiol. 1979 Sep;64(3):428-30. doi: 10.1104/pp.64.3.428.
3
Increase in linolenic Acid is not a prerequisite for development of freezing tolerance in wheat.亚麻酸含量增加不是小麦抗冻性发育的前提条件。
Plant Physiol. 1979 Jan;63(1):5-8. doi: 10.1104/pp.63.1.5.
4
Galactolipid Synthesis in Vicia faba Leaves: II. Formation and Desaturation of Long Chain Fatty Acids in Phosphatidylcholine, Phosphatidylglycerol, and the Galactolipids.蚕豆叶片中的半乳糖脂合成：II. 磷脂酰胆碱、磷脂酰甘油和半乳糖脂中长链脂肪酸的形成与去饱和作用
Plant Physiol. 1976 Feb;57(2):179-84. doi: 10.1104/pp.57.2.179.
5
The role of galactolipids in spinach chloroplast lamellar membranes: I. Partial purification of a bean leaf galactolipid lipase and its action on subchloroplast particles.半乳糖脂在菠菜叶绿体片层膜中的作用：I. 菜豆叶半乳糖脂脂肪酶的部分纯化及其对亚叶绿体颗粒的作用。
Plant Physiol. 1974 May;53(5):699-704. doi: 10.1104/pp.53.5.699.
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The rapid incorporation of phosphate into mitochondrial lipids.磷酸盐迅速掺入线粒体脂质中。
J Biol Chem. 1963 Jan;238:59-63.
7
A comment on the spectrophotometric determination of chlorophyll.关于叶绿素分光光度测定法的一则评论
Biochim Biophys Acta. 1961 Sep 30;52:576-8. doi: 10.1016/0006-3002(61)90418-8.
8
Effect of a substituted pyridazinone, compound BASF 13-338 on membrane lipid synthesis in photosynthetic tissues [proceedings].一种取代哒嗪酮化合物BASF 13 - 338对光合组织中膜脂合成的影响[会议论文集]
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9
Interrelationships between fatty acid biosynthesis and acyl-lipid synthesis in Chlorella vulgaris.小球藻中脂肪酸生物合成与酰基脂质合成之间的相互关系。
Biochem J. 1967 Aug;104(2):486-96. doi: 10.1042/bj1040486.
10
The mechanism of formation of polyunsaturated fatty acids by photosynthetic tissue. The tight coupling of oleate desaturation with phospholipid synthesis in Chlorella vulgaris.光合组织形成多不饱和脂肪酸的机制。小球藻中油酸去饱和与磷脂合成的紧密偶联。
Eur J Biochem. 1969 May 1;9(1):70-8. doi: 10.1111/j.1432-1033.1969.tb00577.x.

分离的叶绿体对外源脂质中亚油酸的去饱和作用。

Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts.

作者信息

Jones A V, Harwood J L

出版信息

Biochem J. 1980 Sep 15;190(3):851-4. doi: 10.1042/bj1900851.

DOI:10.1042/bj1900851
PMID:7470086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162169/
Abstract

When [14C]diacylgalactosylglycerol was added to isolated pea or lettuce chloroplasts linolenate synthesis was seen. The desaturation of [14C]linoleate in diacylgalactosylglycerol to [14C]linolenate was stimulated by the addition of a soluble protein fraction containing lipid-exchange activity. Other [14C]acyl lipids were ineffective, except that [14C]phosphatidylcholine in the presence of UDP-galactose and sn-glycerol 3-phosphate could also supply [14C]linoleate for desaturation. These results are consistent with a role of diacylgalactosylglycerol in linolenate synthesis, as indirectly suggested by labelling experiments.

摘要

当向分离的豌豆或生菜叶绿体中添加[14C]二酰基半乳糖基甘油时，可观察到亚麻酸的合成。含有脂质交换活性的可溶性蛋白质组分的添加刺激了二酰基半乳糖基甘油中[14C]亚油酸去饱和生成[14C]亚麻酸。其他[14C]酰基脂质无效，不过在UDP-半乳糖和sn-甘油3-磷酸存在下的[14C]磷脂酰胆碱也可以提供[14C]亚油酸用于去饱和。这些结果与标记实验间接表明的二酰基半乳糖基甘油在亚麻酸合成中的作用一致。