Alberti S, Stovel R, Herzenberg L A
Cytometry. 1984 Nov;5(6):644-7. doi: 10.1002/cyto.990050614.
Fluorescence-activated cell sorters permit analyses and separation of cell populations based on light scatter and surface immunofluorescence parameters. Since a sorter can deposit individually identifiable cells onto a microscope slide, it was considered of interest to combine the flow measurements with analyses available on cells adhering to a surface as in, for example, morphological studies, cytoplasmic immunofluorescent staining, and mRNA in situ hybridization. A necessary condition for these studies is the preservation of cell structures after sorting. We report here a procedure suitable for this purpose. The most important features of this procedure are A) reducing the saline content of the sorter sheath fluid to about 0.0015 M (one-hundredth that of normal saline) to prevent cell damage due to hypertonicity during drying, and B) coating the substrate with a thin layer of newborn calf serum to promote the adherence of the cells to the substrate during subsequent fixing and staining.
荧光激活细胞分选仪可根据光散射和表面免疫荧光参数对细胞群体进行分析和分离。由于分选仪能够将个体可识别的细胞沉积到显微镜载玻片上，因此将流式测量与附着在表面的细胞的分析方法相结合（例如形态学研究、细胞质免疫荧光染色和mRNA原位杂交）被认为是有意义的。这些研究的一个必要条件是分选后细胞结构的保存。我们在此报告一种适用于此目的的方法。该方法最重要的特点是：A）将分选仪鞘液的盐含量降低至约0.0015 M（生理盐水的百分之一），以防止干燥过程中因高渗性导致细胞损伤；B）在底物上涂覆一层薄薄的新生小牛血清，以促进细胞在后续固定和染色过程中附着到底物上。
