大鼠肝微粒体酸性磷酸酶活性的昼夜节律以及NADH 5α-还原酶对磷酸酶活性的依赖性。
Circadian rhythm of acid phosphatase activity in rat liver microsomes and dependence of NADH 5 alpha-reductase on phosphatase activity.
作者信息
Graef V, Golf S W
出版信息
Hoppe Seylers Z Physiol Chem. 1984 Oct;365(10):1271-6. doi: 10.1515/bchm2.1984.365.2.1271.
The specific activity of acid phosphatase in male and female rats follows a circadian rhythm. Preincubation of liver microsomes with testosterone led to an increase of phosphatase activity and a loss of circadian rhythm. NADH 5 alpha-reductase was inactivated by several animal and bacterial acid and alkaline phosphatases while the acid phosphatase from potatoes was ineffective. The extent of inhibition depends on the course of circadian rhythm of NADH 5 alpha-reductase activity. Preincubation of microsomes in the presence of testosterone inhibited the NADH 5 alpha-reduction of testosterone. No such inhibition was observed after preincubation of microsomes with progesterone.
雄性和雌性大鼠体内酸性磷酸酶的比活性呈现昼夜节律。肝微粒体与睾酮预孵育会导致磷酸酶活性增加以及昼夜节律消失。几种动物和细菌的酸性及碱性磷酸酶可使NADH 5α-还原酶失活，而土豆中的酸性磷酸酶则无此作用。抑制程度取决于NADH 5α-还原酶活性的昼夜节律变化过程。在睾酮存在的情况下对微粒体进行预孵育会抑制睾酮的NADH 5α-还原反应。用孕酮对微粒体进行预孵育后未观察到此类抑制现象。
Zotero 插件