Walters M J
J Assoc Off Anal Chem. 1984 Nov-Dec;67(6):1040-3.
A liquid chromatographic (LC) system, using a bonded cation-exchange column, resolves histamine from histidine and other related compounds. Detection sensitivity and specificity are enhanced by introduction of a post-column reaction with o-phthalaldehyde to form a fluorescent derivative. The limit of detection is 1.5 ng histamine, and the response is linear over a range of 7-750 ng. The average histamine recovery from fortified tuna and mahimahi samples was 98.4% with a relative standard deviation of 2.18%. The results for canned tuna and mahimahi were comparable to those obtained by using the official AOAC method. The LC method requires only simple extraction of the sample before introduction into the LC system, which resolves and detects cadaverine and putrescine in addition to histamine.
一种使用键合阳离子交换柱的液相色谱（LC）系统可将组胺与组氨酸及其他相关化合物分离。通过引入与邻苯二甲醛的柱后反应以形成荧光衍生物，检测灵敏度和特异性得以提高。检测限为1.5 ng组胺，响应在7 - 750 ng范围内呈线性。强化金枪鱼和马希鱼样品中组胺的平均回收率为98.4%，相对标准偏差为2.18%。罐装金枪鱼和马希鱼的检测结果与采用官方AOAC方法获得的结果相当。液相色谱法在将样品引入LC系统之前仅需进行简单提取，该系统除了能分离和检测组胺外，还能分离和检测尸胺和腐胺。
