用于定量分析小麦、玉米及饲料中脱氧雪腐镰刀菌烯醇的改进气相色谱法
Improved gas chromatographic method for quantitation of deoxynivalenol in wheat, corn, and feed.
作者信息
Terhune S J, Nguyen N V, Baxter J A, Pryde D H, Qureshi S A
出版信息
J Assoc Off Anal Chem. 1984 Nov-Dec;67(6):1102-4.
A gas chromatographic (GC) method is described to determine deoxynivalenol in wheat and corn at levels as low as 20 ppb. Ground samples are extracted with water, adsorbed onto a Clin Elut column, extracted with ethyl acetate, and passed through a silica gel Sep-Pak cartridge. The final extract is then derivatized with N-heptafluorobutyrylimidazole and quantitated by GC using an electron capture detector. Recoveries are greater than 85% for spiked samples at levels of 50-1000 ppb. Results for wheat, corn, and mixed feed samples are given as well as the results of an interlaboratory study on a naturally contaminated wheat sample.
描述了一种气相色谱（GC）方法，用于测定小麦和玉米中低至20 ppb水平的脱氧雪腐镰刀菌烯醇。将磨碎的样品用水提取，吸附到Clin Elut柱上，用乙酸乙酯提取，然后通过硅胶Sep-Pak柱。最后提取物用N-七氟丁酰咪唑衍生化，并使用电子捕获检测器通过GC进行定量。对于加标水平为50-1000 ppb的样品，回收率大于85%。给出了小麦、玉米和混合饲料样品的结果以及对天然污染小麦样品的实验室间研究结果。
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