同时测定血浆和心肌中的利多卡因及其代谢物。

Simultaneous determination of lidocaine and its metabolites in plasma and myocardium.

作者信息

Luzzi F A, Wenger T L, Klinger J K, Barchowsky A, Strauss H C

出版信息

J Chromatogr. 1984 Nov 28;311(2):291-9. doi: 10.1016/s0378-4347(00)84722-2.

PMID:6520176

Abstract

No validated method exists for measuring lidocaine and its metabolites in myocardial tissue. We modified a previously described high-performance liquid chromatographic assay and applied it to plasma and to homogenized myocardial samples obtained from dogs that had received lidocaine by a double-infusion technique. Recovery of lidocaine, monoethylglycylxylidide and glycylxylidide after homogenization and extraction is reported. Assay variability, sensitivity and linearity over a wide range of sample sizes are also described. The results obtained with high-performance liquid chromatographic analysis are compared to quantitation of 14C-labeled lidocaine plus metabolites measured by an oxidation-scintillation technique. Myocardium to plasma partition coefficients for lidocaine, monoethylglycylxylidide and glycylxylidide were 2.16, 4.27, and 2.91, respectively.

摘要

目前尚无经过验证的方法可用于测量心肌组织中的利多卡因及其代谢产物。我们对先前描述的高效液相色谱分析方法进行了改进，并将其应用于通过双输注技术接受利多卡因的犬只的血浆和匀浆心肌样本。报告了匀浆和提取后利多卡因、单乙基甘氨酰二甲苯和甘氨酰二甲苯的回收率。还描述了在广泛的样本量范围内的分析变异性、灵敏度和线性。将高效液相色谱分析获得的结果与通过氧化闪烁技术测量的14C标记利多卡因加代谢产物的定量结果进行了比较。利多卡因、单乙基甘氨酰二甲苯和甘氨酰二甲苯的心肌与血浆分配系数分别为2.16、4.27和2.91。