利用场强梯度在DNA测序凝胶中形成均匀间隔的条带模式。

Uniformly spaced banding pattern in DNA sequencing gels by use of field-strength gradient.

作者信息

Olsson A, Moks T, Uhlén M, Gaal A B

出版信息

J Biochem Biophys Methods. 1984 Nov;10(1-2):83-90. doi: 10.1016/0165-022x(84)90053-8.

PMID:6520329

Abstract

A method is described for casting and operating ultrathin wedge-shaped field-strength gradient gels for DNA sequencing. One of the gradients results in a uniformly-spaced oligonucleotide banding pattern from 60 to 300 nucleotides in a 53 cm long 4% polyacrylamide gel. From these field-strength gradient gels, more sequencing data can be obtained with greater confidence than from gels with uniform thickness. The gel-casting method is fast and simple. At 2.5 kV two gels can be run simultaneously in 2 h with the same power supply.

摘要

本文描述了一种用于浇铸和操作超薄楔形场强梯度凝胶以进行DNA测序的方法。其中一种梯度在53厘米长的4%聚丙烯酰胺凝胶中产生60至300个核苷酸的均匀间隔寡核苷酸条带模式。与厚度均匀的凝胶相比，从这些场强梯度凝胶中可以获得更可靠的更多测序数据。凝胶浇铸方法快速且简单。在2.5 kV电压下，使用同一电源可在2小时内同时运行两块凝胶。