Depaolis A M, Britt T E, Holman A J, McGonigle E J, Kaplan G, Davies W C
J Pharm Sci. 1984 Nov;73(11):1650-1. doi: 10.1002/jps.2600731143.
A rapid and sensitive high-performance liquid chromatographic quantitation of meclocycline (I) in a cream formulation is described. The acidified methanolic extract of the sample was diluted with mobile phase and analyzed on a reverse-phase column by using a mobile phase consisting of EDTA buffer (pH 6.6)-tetrahydrofuran (85:15 v/v). The method gave linear, quantitative, and reproducible results with a detection limit of 0.4 ppm for meclocycline.
本文描述了一种快速灵敏的高效液相色谱法，用于定量测定乳膏制剂中的甲氯环素（I）。样品的酸化甲醇提取物用流动相稀释，然后在反相柱上进行分析，流动相由EDTA缓冲液（pH 6.6）-四氢呋喃（85:15 v/v）组成。该方法给出了线性、定量且可重现的结果，甲氯环素的检测限为0.4 ppm。
