[Production of monoclonal antibodies specific to human lung cancer--squamous cell carcinoma and adenocarcinoma].

UNLABELLED

Hybridomas secreting monoclonal antibodies specific for human lung cancer were produced by fusing immunized mouse spleen cells with mouse myeloma line 653.

METHODS

BALB/c mice were hyperimmunized with two different histological types of human lung cancer (squamous cell carcinoma and adenocarcinoma) obtained from surgery. An immunocytoadherence test was used to select hybridomas secreting antibodies that bound to the patient's lung tumor but did not bind to a B-lymphoblastoid cell line derived from the same patient. Five stable antibody-producing hybrids (2C6, 5D7 and 5E8 derived from squamous cell carcinoma, 5C7 and 2B7 derived from adenocarcinoma) have been established and cloned.

RESULTS

The antibodies bound to other lung tumors and established lung tumor cell lines of the same histological type. Also, some significant reactivity was observed with large cell carcinoma, but the antibodies did not react with small cell carcinoma of the lung, bronchiolo-alveolar cell carcinoma, cancer of the stomach and esophagus, melanoma, several types of leukemias, normal human lung tissue, fibroblasts, or erythrocytes of type A, B or O. Two of the five antibodies, 5C7 and 5E8 cross-reacted with one breast cancer obtained from surgery and 5C7 also cross-reacted with one melanoma biopsy specimen. Moreover, the antibodies have been characterized according to their light and heavy chain isotypes by ouchterlony and radioimmunoassay.