Kyoizumi S, Akiyama M, Kouno N, Kobuke K, Hakoda M, Jones S L, Yamakido M
Cancer Res. 1985 Jul;45(7):3274-81.
Three immunoglobulin G1 monoclonal antibodies, LuCa2, LuCa3, and LuCa4, were produced by fusing murine myeloma NS1 cells with splenocytes obtained from a BALB/c mouse immunized with SK-MES1 cells derived from human squamous cell carcinoma of the lung. These three monoclonal antibodies were shown to recognize different protein antigens on SK-MES1 cells by indirect immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. While the pattern of cell line distribution of antigens recognized by these antibodies was not tumor type specific, their reactivity with tissue and pleural effusion was much more informative than with cell lines. The presence of target antigens in vivo was analyzed by immunoperoxidase staining of frozen tissue sections and immunofluorescence staining of tumor cells in pleural effusions. LuCa2 antibody was reactive with lung squamous carcinoma and adenocarcinoma tumor tissues and pleural effusions, but only infrequently with those of small cell carcinoma. This antibody was also reactive with many tumor tissues from other organs as well as with various normal tissues, including alveoli and bronchus. LuCa3 and LuCa4 antibodies reacted with lung squamous carcinoma in tissues and pleural effusions, but not with lung adenocarcinoma nor with small cell carcinoma. These two antibodies reacted only weakly with normal squamous tissues of the esophagus, skin, and cervix uteri, but not with various other normal tissues. Moreover, LuCa3 had weak reactivity with squamous cell carcinoma tissue of tongue and esophagus, whereas LuCa4 had no reactivity with nonpulmonary tumor tissues. LuCa3 and LuCa4 antibodies should be of clinical interest, because our data suggest that these antibodies may be potentially useful for the diagnosis of the histological type of lung tumor cells in both cancer tissue and pleural effusions.
通过将小鼠骨髓瘤NS1细胞与用源自人肺鳞状细胞癌的SK-MES1细胞免疫的BALB/c小鼠的脾细胞融合，制备了三种免疫球蛋白G1单克隆抗体，即LuCa2、LuCa3和LuCa4。通过间接免疫沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，这三种单克隆抗体可识别SK-MES1细胞上不同的蛋白质抗原。虽然这些抗体识别的抗原的细胞系分布模式并非肿瘤类型特异性的，但它们与组织和胸腔积液的反应性比与细胞系的反应性更具信息价值。通过对冷冻组织切片进行免疫过氧化物酶染色以及对胸腔积液中的肿瘤细胞进行免疫荧光染色，分析了体内靶抗原的存在情况。LuCa2抗体与肺鳞状癌和腺癌肿瘤组织及胸腔积液有反应，但与小细胞癌的反应较少。该抗体还与来自其他器官的许多肿瘤组织以及包括肺泡和支气管在内的各种正常组织有反应。LuCa3和LuCa4抗体与组织和胸腔积液中的肺鳞状癌有反应，但与肺腺癌和小细胞癌无反应。这两种抗体仅与食管、皮肤和子宫颈的正常鳞状组织有微弱反应，但与其他各种正常组织无反应。此外，LuCa3与舌和食管的鳞状细胞癌组织有微弱反应，而LuCa4与非肺肿瘤组织无反应。LuCa3和LuCa4抗体可能具有临床意义，因为我们的数据表明，这些抗体可能对诊断癌组织和胸腔积液中肺肿瘤细胞的组织学类型有潜在用途。
