Kuznetsov I A, Kargov S I, Kozlov A G
Mol Biol (Mosk). 1984 Nov-Dec;18(6):1583-9.
Ion exchangers with various capacities (0.1-0.2 mg-equiv/g of dry gel) are synthesized by means of immobilization of DNA in polyacrylamide gel. Exchanges of alkali metal cations and ammonium are studied on these exchangers and selectively coefficients are determined. The following selectivity series of immobilized DNA in reference to the above-mentioned cations is stated: Li+ greater than or equal to NH4+ greater than or equal to Cs+ greater than Rb+ greater than K+ greater than or equal to Na+. The peculiar properties of Li+ and NH4+ in this series are noted and a possible explanation of this fact is offered. A supposition regarding the reduced activity of water in the polyacrylamide gel containing DNA is made.
通过将DNA固定在聚丙烯酰胺凝胶中来合成具有不同容量（0.1 - 0.2毫克当量/克干凝胶）的离子交换剂。研究了这些离子交换剂上碱金属阳离子和铵的交换情况，并测定了选择性系数。得出了固定化DNA相对于上述阳离子的以下选择性序列：Li + ≥ NH4 + ≥ Cs + ＞ Rb + ＞ K + ≥ Na + 。指出了该序列中Li + 和NH4 + 的特殊性质，并对此现象给出了一种可能的解释。提出了关于含DNA的聚丙烯酰胺凝胶中水活性降低的假设。
