一种用于检测鸡新城疫病毒保护性抗体水平的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay that measures protective antibody levels to Newcastle disease virus in chickens.
作者信息
Wilson R A, Perrotta C, Frey B, Eckroade R J
出版信息
Avian Dis. 1984 Oct-Dec;28(4):1079-85.
PMID:6525128
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to measure antibodies to Newcastle disease virus (NDV) in chickens. Chickens 6 to 33 weeks old, with a variety of vaccination histories, were bled before challenge with a velogenic strain of NDV. Fourteen days post-challenge, 63 of the 73 challenged birds had survived. ELISA results of pre-challenge sera corresponded directly with survival rates of birds challenged with NDV.
摘要
开发了一种酶联免疫吸附测定法（ELISA）来检测鸡体内新城疫病毒（NDV）的抗体。对6至33周龄、有不同疫苗接种史的鸡，在用强毒株新城疫病毒攻毒前采血。攻毒后14天，73只攻毒鸡中有63只存活。攻毒前血清的ELISA结果与新城疫病毒攻毒鸡的存活率直接相关。
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