Wulf H C, Wettermark G, Husum B
Biomed Biochim Acta. 1984;43(10):1173-7.
ATP measurements were performed on perfusates from muscles in anaesthetized pigs in vivo. Samples were obtained every 5 min by perfusion of a double-walled, fenestrated cannula inserted in the muscle. Five pigs served as controls and in 6 animals the adductor muscles of the thigh were electrically stimulated to contract each second for a period of 30 min. ATP was measured by the firefly bioluminescence technique. Immediately after insertion of the cannula ATP was high in the perfusate probably due to the physical damage to the cells and capillaries. After 50 min ATP reached a rather low level. Compared to controls, ATP in centrifuged, cell-free perfusate was unchanged during 30 min of muscle activity and for 60 min thereafter. In cell-containing samples ATP was slightly increased during the muscle activity period, probably due to damage from the cannula. It was concluded that, by this method, no indication was found of an association of muscular activity and release of ATP into the extracellular space.
对麻醉猪体内肌肉的灌注液进行了ATP测量。通过插入肌肉的双壁多孔套管灌注，每5分钟采集一次样本。5只猪作为对照，6只动物的大腿内收肌每秒接受电刺激收缩，持续30分钟。采用萤火虫生物发光技术测量ATP。插管后立即，灌注液中的ATP含量很高，这可能是由于细胞和毛细血管受到物理损伤所致。50分钟后，ATP达到相当低的水平。与对照组相比，在肌肉活动的30分钟及之后的60分钟内，离心后的无细胞灌注液中的ATP没有变化。在含有细胞的样本中，肌肉活动期间ATP略有增加，这可能是由于插管造成的损伤。得出的结论是，通过这种方法，未发现肌肉活动与ATP释放到细胞外空间之间存在关联的迹象。
