从印度眼镜蛇（眼镜蛇属）毒液中分离出的细胞毒素IIa的氨基酸序列。

Amino acid sequence of cytotoxin IIa isolated from the venom of the Indian cobra (Naja naja).

作者信息

Kaneda N, Takechi M, Sasaki T, Hayashi K

出版信息

Biochem Int. 1984 Nov;9(5):603-10.

Abstract

A cytotoxic basic polypeptide, designated as cytotoxin IIa, was purified to homogeneous state from the venom of the Indian cobra (Naja naja) by a combination of gel filtration on Sephadex G-50, CM-cellulose chromatography, and fast protein liquid chromatography. Cytotoxin IIa is a single polypeptide consisting of 60 amino acid residues with four intramolecular disulfide linkages. The toxin showed high cytotoxicity toward Yoshida sarcoma and ascites hepatoma cells as did cytotoxins I and II isolated from the same venom. Analysis of the amino acid sequence revealed that cytotoxin I, IIa, and II are highly homologous in their primary structures and that cytotoxin IIa differs from cytotoxin I only in having Phe 25 and Val 52 in place of Tyr 25 and Glu 52 residues.

摘要

一种细胞毒性碱性多肽，命名为细胞毒素IIa，通过Sephadex G - 50凝胶过滤、CM - 纤维素色谱和快速蛋白质液相色谱相结合的方法，从印度眼镜蛇（Naja naja）毒液中纯化至同质状态。细胞毒素IIa是一种由60个氨基酸残基组成的单一多肽，具有四个分子内二硫键。该毒素对吉田肉瘤和腹水肝癌细胞显示出高细胞毒性，与从同一毒液中分离出的细胞毒素I和II相同。氨基酸序列分析表明，细胞毒素I、IIa和II在其一级结构上高度同源，并且细胞毒素IIa与细胞毒素I的不同之处仅在于其第25位的苯丙氨酸和第52位的缬氨酸取代了酪氨酸25和谷氨酸52残基。