Takechi M, Tanaka Y, Hayashi K
Biochem Int. 1987 Jan;14(1):145-52.
A less-cytotoxic polypeptide, designated as LCBP, was isolated from the venom of Naja naja by gel filtration on Sephadex G-50 followed by CM-cellulose chromatography. The cytotoxicity toward Yoshida sarcoma cells and lethal toxicity toward mice of LCBP were both one order of magnitude lower than that of cytotoxins and that of toxin A, respectively. LCBP is a single polypeptide consisting of 61 amino acid residues with four intramolecular disulfide linkages, and the amino acid sequence is the same as that of cardiotoxin-like basic polypeptide (CLBP) isolated from the venom of Naja naja atra. This is the first time that the same polypeptides were isolated from different cobra venoms.
一种细胞毒性较低的多肽，命名为LCBP，通过在Sephadex G - 50上进行凝胶过滤，随后进行CM - 纤维素色谱法，从眼镜蛇毒液中分离得到。LCBP对吉田肉瘤细胞的细胞毒性和对小鼠的致死毒性分别比细胞毒素和毒素A低一个数量级。LCBP是一种由61个氨基酸残基组成的单一多肽，具有四个分子内二硫键，其氨基酸序列与从舟山眼镜蛇毒液中分离得到的类心脏毒素碱性多肽（CLBP）相同。这是首次从不同眼镜蛇毒液中分离出相同的多肽。
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