Hardee G H, Fleitman J S, Otagiri M, Perrin J H
Biopharm Drug Dispos. 1984 Oct-Dec;5(4):307-14. doi: 10.1002/bdd.2510050403.
Flow microcalorimetry was used to estimate primary binding constants for drug-albumin interactions. Measurements of heat of reaction at two temperatures illustrated the danger of extrapolation for pharmacokinetic purposes of measurements made at temperatures other than 37 degrees. The method could be used to predict competition between two drugs for a single binding site. Major advantages over spectroscopic techniques included direct determinations of thermodynamic parameters, and the use of physiological concentrations of albumin.
流动微量量热法用于估算药物与白蛋白相互作用的一级结合常数。在两个温度下对反应热的测量表明，将在37摄氏度以外的温度下进行的测量用于药代动力学目的进行外推存在风险。该方法可用于预测两种药物对单个结合位点的竞争情况。与光谱技术相比，主要优点包括直接测定热力学参数以及使用生理浓度的白蛋白。
