Tamai Y, Waters R S, Asanuma H
Brain Res. 1984 Dec 10;323(2):360-4. doi: 10.1016/0006-8993(84)90314-7.
The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20% solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.
运用电生理和解剖学相结合的技术，对猫丘脑腹外侧核（VL）和腹后外侧核（VPL）之间的边界区域（VL-VPL）的输入进行了研究。该边界区域内的神经元接受躯体传入输入，并投射到具有相似感受野的运动皮层区域。在本研究中，我们提出了一个问题，即VL-VPL边界内的神经元是否接受来自薄束核（DCN）的输入。为了回答这个问题，我们在向运动皮层进行皮层内微刺激（ICMS）的同时，将第二个电极插入充满溶解于氯化钾的20%辣根过氧化物酶（HRP）溶液的VL-VPL边界，用于记录逆向激活的神经元。当遇到逆向激活的神经元且该神经元对自然外周刺激有反应时，通过记录电极进行HRP离子电渗注射。在48 - 72小时的存活期后，处死猫，并按照哈迪和海默的方法处理脑组织。在所有注射动物的尾侧楔束核（CCN）中发现了标记的细胞体。这些结果表明，CCN中的神经元投射到VL-VPL中的细胞，而VL-VPL中的细胞又投射到运动皮层。
