Vennström B, Kahn P, Adkins B, Enrietto P, Hayman M J, Graf T, Luciw P
EMBO J. 1984 Dec 20;3(13):3223-9. doi: 10.1002/j.1460-2075.1984.tb02282.x.
A murine retrovirus which expresses the avain v-myc OK10 oncogene was constructed. The virus, denoted MMCV, readily transforms fibroblasts of established lines, such as mouse NIH/3T3 and rat 208F cells, to anchorage-independent growth in agarose. The virus also transforms primary mouse cells: (i) virus-infected macrophages are induced to form large colonies in semi-solid media, and can easily be expanded into mass cultures; (ii) MMCV-infected fibroblastic cells from mouse limb buds undergo morphological transformation and grow in semi-solid medium. MMCV thus transforms both mouse fibroblastic cells and macrophages in vitro, in a fashion similar to the v-myc-containing avian viruses in chicken cells. The possibility of introducing a transforming myc gene into mammalian cells by virus infection provides a novel approach for studying the mechanism of myc transformation in cells from many lineages.
构建了一种表达禽v-myc OK10癌基因的鼠逆转录病毒。该病毒命名为MMCV，能轻易地将诸如小鼠NIH/3T3和大鼠208F细胞等已建立细胞系的成纤维细胞转化为在琼脂糖中不依赖贴壁的生长状态。该病毒还能转化原代小鼠细胞：（i）病毒感染的巨噬细胞在半固体培养基中被诱导形成大菌落，并能轻易地扩展为大量培养物；（ii）来自小鼠肢芽的MMCV感染的成纤维细胞发生形态转化并在半固体培养基中生长。因此，MMCV在体外能转化小鼠成纤维细胞和巨噬细胞，其方式类似于含v-myc的禽病毒在鸡细胞中的作用。通过病毒感染将转化性myc基因导入哺乳动物细胞的可能性为研究来自多种谱系的细胞中myc转化机制提供了一种新方法。
