Wright J F, Smith S, Micklem L R, James K
Dev Biol Stand. 1984;57:17-25.
The production of relatively large amounts of pure monoclonal antibodies (MAb) has facilitated the development of MAb-based immunometric assays for a variety of clinically important analytes. 'Two-site' heterogeneous assays are now available which incorporate a labelled MAb and a second, different MAb coupled to a solid support. These assays possess certain advantages over the corresponding immunoassays including speed, precision, working range and specificity. They are largely dependent on two specific molecular interactions (labelled MAb-antigen; antigen--solid support MAb) and thus one might expect them to be particularly sensitive to assay conditions. With reference to two MAb--based two-site immunoradiometric assays (for human growth hormone and prolactin) which are being developed in this laboratory we wish to report the effect of various conditions including pH, ionic strength, buffer species etc. on assay response in order to emphasize the need for careful optimisation of monoclonal antibody based assays.
大量生产纯单克隆抗体（MAb）推动了基于MAb的免疫分析方法的发展，用于检测多种临床上重要的分析物。现在已有“双位点”非均相分析方法，该方法结合了标记的MAb和与固相支持物偶联的第二种不同的MAb。这些分析方法相对于相应的免疫分析方法具有某些优势，包括速度、精密度、工作范围和特异性。它们在很大程度上依赖于两种特定的分子相互作用（标记的MAb-抗原；抗原-固相支持物MAb），因此人们可能预期它们对分析条件特别敏感。关于本实验室正在开发的两种基于MAb的双位点免疫放射分析方法（用于检测人生长激素和催乳素），我们希望报告包括pH值、离子强度、缓冲液种类等各种条件对分析响应的影响，以强调对基于单克隆抗体的分析方法进行仔细优化的必要性。
