Thorpe R, Perry M J, Callus M, Gaffney P J, Spitz M
Hybridoma. 1984 Winter;3(4):381-5. doi: 10.1089/hyb.1984.3.381.
We describe the use of the single shot intrasplenic immunization technique as a particularly effective procedure for the production of specific monoclonal antibodies against a high molecular weight antigen. We found that with this technique several different, completely specific monoclonal antibodies could be produced against high molecular weight crosslinked fibrin degradation products. These results contrasted with those obtained using conventional multidose immunization, which only produced monoclonal antibodies that were cross-reactive with fibrinogen and/or noncrosslinked fibrin degradation products.
我们描述了单次脾内免疫技术的应用，该技术是一种特别有效的方法，可用于生产针对高分子量抗原的特异性单克隆抗体。我们发现，使用这种技术，可以产生几种针对高分子量交联纤维蛋白降解产物的不同的、完全特异性的单克隆抗体。这些结果与使用传统多剂量免疫获得的结果形成对比，传统多剂量免疫仅产生与纤维蛋白原和/或非交联纤维蛋白降解产物交叉反应的单克隆抗体。
