Inaba M, Hatayama T, Wada M, Yukioka M
J Biochem. 1984 Nov;96(5):1337-42. doi: 10.1093/oxfordjournals.jbchem.a134961.
When rat liver nuclei were digested with nuclease, we found that the chromatin-bound RNA polymerase II was liberated as two distinct complexes, peak 1 and peak 2, which seemed to reflect different functional states in cell nuclei. We further examined their occurrence in nuclear digests of various tissues of rats and the following results were obtained. Upon digestion with micrococcal nuclease of nuclei from brain, spleen, testis and kidney, chromatin-bound RNA polymerase II was liberated as two distinct forms which sedimented differently in a sucrose density gradient. The sedimentation rate of peak 1 varied depending on the tissue nuclei examined. After high salt or RNase treatment of the nuclear digests, peak 1 from liver, brain, spleen and testis nuclei showed the same sedimentation rate as did kidney peak 1, the rate for which remained unchanged by these treatments. The results suggested that peak 1 complexes from various tissue nuclei had basically the same structural organization, and we confirmed this by electrophoretic studies on RNase-treated liver and kidney nuclear digests. Peak 2 from various tissue nuclei exhibited identical sedimentation rates. Thus, the chromatin-bound RNA polymerase II seems to exist commonly in two distinct states in cell nuclei of rats.
当用核酸酶消化大鼠肝细胞核时，我们发现与染色质结合的RNA聚合酶II以两种不同的复合物形式释放出来，即峰1和峰2，这似乎反映了细胞核中的不同功能状态。我们进一步研究了它们在大鼠各种组织的核消化物中的存在情况，得到了以下结果。在用微球菌核酸酶消化脑、脾、睾丸和肾的细胞核后，与染色质结合的RNA聚合酶II以两种不同的形式释放出来，它们在蔗糖密度梯度中的沉降方式不同。峰1的沉降速率因所检测的组织细胞核而异。对核消化物进行高盐或核糖核酸酶处理后，肝、脑、脾和睾丸细胞核的峰1与肾峰1的沉降速率相同，且该速率在这些处理后保持不变。结果表明，来自各种组织细胞核的峰1复合物基本具有相同的结构组织，我们通过对经核糖核酸酶处理的肝和肾核消化物的电泳研究证实了这一点。来自各种组织细胞核的峰2表现出相同的沉降速率。因此，与染色质结合的RNA聚合酶II似乎在大鼠细胞核中普遍以两种不同的状态存在。
