通过固定化金属亲和色谱法分离人血清蛋白

Fractionation of human serum proteins by immobilized metal affinity chromatography.

作者信息

Andersson L

出版信息

J Chromatogr. 1984 Dec 19;315:167-74. doi: 10.1016/s0021-9673(01)90734-4.

Abstract

Human serum was fractionated on zinc and cadmium iminodiacetate and tris(carboxymethyl)ethylenediamine chelates. Zinc iminodiacetate was found to be useful in the isolation of hemopexin and alpha 2-macroglobulin. The chromatographic behaviour of serum proteins on zinc iminodiacetate could be modulated by the nature of the eluent; a sulphate-containing solvent increased the binding of serum proteins to immobilized metal ions.

摘要

人血清在亚氨基二乙酸锌和三（羧甲基）乙二胺螯合物上进行分离。发现亚氨基二乙酸锌可用于分离血红素结合蛋白和α2-巨球蛋白。血清蛋白在亚氨基二乙酸锌上的色谱行为可通过洗脱液的性质进行调节；含硫酸盐的溶剂会增加血清蛋白与固定化金属离子的结合。