Godard J, Coulon G, Monnier G, Rouillon D, Jacquet G, Bourghli A, Steimle R
Neurochirurgie. 1984;30(6):407-16.
An experimental study of nerve regeneration in rats was realized after sectioning 8 mm of the sciatic nerve and interpositioning a vein-graft to replace the portion of nerve which has been removed. The vein-graft was taken at the neck level, far away from the point of suture between vein and nerve, a procedure which seems to prevent the problems of scabbing. A histological and electrophysiological analysis was carried out on 16 grafts, over periods ranging from 4 weeks to 4 months. The nerve and venous dissections were realized under a stereoscopic microscope. A certain degree of amyotrophy appeared in the external-posterior region of the thigh, directly related to the muscular dissection and the partial paralysis caused by the section of the common peroneal nerve. The electrophysiological recordings in vivo provide proof of nerve regeneration but reveal the absence of any correlation between the time of recovery and the time of induction. Examination under the light microscope confirms the regeneration of the nerve inside of the vein-graft, with an augmentation of myelin sheaths, which increase with time both in number and in thickness. The vein-wall acts as a guide but the passage of a few nervous filaments outside of the graft itself is observed. Examination under the electron microscope furnishes important information on myelin sheaths. The results are of excellent quality, but to better appreciate and evaluate them, we envisage a further study, this time to be carried out on another animal species.
在切断大鼠坐骨神经8毫米并插入静脉移植物以替代已切除的神经部分后，对大鼠神经再生进行了一项实验研究。静脉移植物取自颈部水平，远离静脉与神经的缝合点，这一操作似乎可避免结痂问题。对16个移植物进行了为期4周到4个月的组织学和电生理分析。神经和静脉解剖在立体显微镜下进行。大腿后外侧区域出现了一定程度的肌萎缩，这与肌肉解剖以及腓总神经切断导致的部分麻痹直接相关。体内电生理记录证明了神经再生，但显示恢复时间与诱导时间之间不存在任何相关性。光学显微镜检查证实了静脉移植物内神经的再生，髓鞘有所增加，其数量和厚度均随时间增加。静脉壁起到了引导作用，但观察到有一些神经细丝穿出移植物本身。电子显微镜检查提供了有关髓鞘的重要信息。结果质量很好，但为了更好地理解和评估它们，我们设想进一步开展研究，这次将在另一种动物身上进行。
