Greenquist A C, Wyatt J L, Guatelli J C, Shohet S B
Prog Clin Biol Res. 1978;20:1-24.
Phosphorylation of spectrin in whole cells occurs on a single tryptic peptide and indicates a specific and limited modification of spectrin. Phosphorylation of spectrin in soluble extracts and isolated membranes is less specific. Diamide is an inhibitor of erythrocyte spectrin kinase and casein kinase. This agent also inhibits the restoration of biconcave morphology to metabolically depleted erythrocytes reincubated with adenosine.
全细胞中血影蛋白的磷酸化发生在单一胰蛋白酶肽段上，表明血影蛋白发生了特定且有限的修饰。可溶性提取物和分离膜中血影蛋白的磷酸化特异性较低。二酰胺是红细胞血影蛋白激酶和酪蛋白激酶的抑制剂。该试剂还抑制代谢耗尽的红细胞在与腺苷一起再孵育时双凹形态的恢复。
