[用磷脂恢复新城疫病毒中血凝素 - 神经氨酸酶的生物活性]
[Restoration of the biological activity of hemagglutinin--neuraminidase in NDV with phospholipids].
作者信息
Vasileva L, Dumanova L
出版信息
Vet Med Nauki. 1984;21(9):33-7.
PMID:6528478
Abstract
The phospholipids in the membrane of the Newcastle disease virus were hydrolysed by means of the phospholipase enzyme C, whereas the infectious titer of the virus did not change, however its neuraminidase activity dropped. It was found that sphingomyelin in conc. of 250 micrograms/cm3 or lipids of the Newcastle disease virus membrane added to phospholipase C-treated hemagglutinin-neuraminidase restored its activity. The phospholipid 'phosphatidiletanolamine' could not restore the activity of the virus neuraminidase.
摘要
新城疫病毒膜中的磷脂通过磷脂酶C进行水解，然而病毒的感染滴度没有变化，但其神经氨酸酶活性下降。研究发现，浓度为250微克/立方厘米的鞘磷脂或添加到经磷脂酶C处理的血凝素-神经氨酸酶中的新城疫病毒膜脂质可恢复其活性。磷脂“磷脂酰乙醇胺”不能恢复病毒神经氨酸酶的活性。
相似文献
1
[Restoration of the biological activity of hemagglutinin--neuraminidase in NDV with phospholipids].[用磷脂恢复新城疫病毒中血凝素 - 神经氨酸酶的生物活性]
Vet Med Nauki. 1984;21(9):33-7.
2
The pathogenesis of Newcastle disease: a comparison of selected Newcastle disease virus wild-type strains and their infectious clones.新城疫的发病机制：选定的新城疫病毒野生型毒株及其感染性克隆的比较。
Virology. 2006 Sep 30;353(2):333-43. doi: 10.1016/j.virol.2006.06.013. Epub 2006 Jul 24.
3
Topological location and biological significance of phospholipids in the membrane of Newcastle disease virus. Hydrolysis of phospholipids in intact virion with pure phospholipases A2, C, and D.
J Biochem. 1982 Aug;92(2):575-83. doi: 10.1093/oxfordjournals.jbchem.a133966.
4
Evaluation of Newcastle disease virus chimeras expressing the Hemagglutinin-Neuraminidase protein of velogenic strains in the context of a mesogenic recombinant virus backbone.在中等毒力重组病毒骨架背景下，对表达速发型毒株血凝素神经氨酸酶蛋白的新城疫病毒嵌合体进行评估。
Virus Res. 2007 Nov;129(2):182-90. doi: 10.1016/j.virusres.2007.07.008. Epub 2007 Aug 24.
5
[Expression of a cloned hemagglutinin-neuraminidase gene from Newcastle disease virus in murine myeloma cells].
Mol Biol (Mosk). 2001 Sep-Oct;35(5):920-7.
6
7
8
The activity of Newcastle disease virus-envelope proteins after treatment with detergents.用去污剂处理后新城疫病毒包膜蛋白的活性。
Experientia. 1975 Dec 15;31(12):1397-8. doi: 10.1007/BF01923207.
9
The fusion promotion activity of the NDV HN protein does not correlate with neuraminidase activity.新城疫病毒血凝素-神经氨酸酶（NDV HN）蛋白的融合促进活性与神经氨酸酶活性无关。
Virology. 1993 Oct;196(2):831-4. doi: 10.1006/viro.1993.1541.
10
Contribution of HN protein length diversity to Newcastle disease virus virulence, replication and biological activities.HN 蛋白长度多样性对新城疫病毒毒力、复制和生物学活性的贡献。
Sci Rep. 2016 Nov 11;6:36890. doi: 10.1038/srep36890.
Zotero 插件