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[奶牛地方流行性白血病中血清与乳清的琼脂凝胶沉淀法(AGP)比较研究]

[Comparative studies of blood sera and lactosera by agar gel precipitation (AGP) in enzootic leukemia in cows].

作者信息

Arnaudov Kh, Boiadzhieva L

出版信息

Vet Med Nauki. 1984;21(9):7-11.

PMID:6528481
Abstract

Studied were a total of 70 samples of blood and corresponding lactosera taken from lactating cows that were positive for leukosis on three farms with records for the disease. The lactosera were obtained from the milk samples through treatment in two ways--with calcium chloride and with chymosin. The experiments were carried out with untreated blood sera and with the lactosera obtained in both ways. Parallel to these, blood sera and lactosera were both studied in a concentrated state after being deeply frozen and centrifugated. The agar gel precipitation test (AGPT) was employed. It was concluded that the procedure described was a readily applicable method for laboratory use in the way of establishing enzootic leukosis, making unnecessary the blood sampling on a mass scale as undesirable from an economic and epizootic viewpoint.

摘要

共研究了来自三个有白血病记录农场的70份泌乳期患白血病阳性奶牛的血液样本及相应的乳清样本。乳清通过两种方式从牛奶样本中获得——用氯化钙处理和用凝乳酶处理。实验使用未处理的血清以及通过两种方式获得的乳清进行。与此同时,血液血清和乳清在深度冷冻和离心后均以浓缩状态进行研究。采用琼脂凝胶沉淀试验(AGPT)。得出的结论是,所描述的程序是一种易于应用于实验室的方法,用于确定地方性白血病,从经济和动物流行病学角度来看,无需进行大规模采血。

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