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[Highly-efficient apparatus for two-dimensional gel electrophoresis].

作者信息

Shishkin S S, Volodina T V, Pontriagina N Iu, Debov S S

出版信息

Vopr Med Khim. 1984;30(6):126-31.

PMID:6528532
Abstract

An apparatus for two-dimensional gel electrophoresis described enabled to analyze 18 samples simultaneously. The apparatus included a device for electrophoresis in small columns of a gel (A), a device (B) for formation of 18 gel plates and a device (C) for staining and washing of the plates. In order to choose the most optimal conditions for fractionation all the parts of the apparatus are separated by reciprocally perpendicular partitions into sections, which allow to use various buffer systems simultaneously. Results of heart muscle proteins fractionation, carried out in the apparatus on the basis of O'Farrell and Laemmly procedures, are presented.

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