Eguchi M, Ueda K, Yamashita M
Biochem Genet. 1984 Dec;22(11-12):1093-102. doi: 10.1007/BF00499634.
Many electrophoretic variants of hemolymph inhibitors of proteases from Aspergillus melleus and pancreatic alpha-chymotrypsin were found using 126 silkworm strains. Six inhibitors of the fungal protease were detected and eight of chymotrypsin; the distribution of inhibitors among Japanese, Chinese, and European races was investigated. Comparison of electrophoretic patterns from F1 hybrids and parents showed that the offspring produce inhibitors of both parental types. Segregation in F2 and backcrossing suggest that the expression of each inhibitor is controlled in most cases by a pair of alleles which are responsible for strong and null bands. Two bands of fungal protease inhibitors C and D were controlled by codominant alleles. These results suggest that polymorphism of hemolymph protease inhibitors in the silkworm would be a useful experimental system for the study of the genetic control of protease inhibitors.
利用126个家蚕品系，发现了来自黑曲霉的血淋巴蛋白酶抑制剂和胰凝乳蛋白酶的许多电泳变体。检测到6种真菌蛋白酶抑制剂和8种胰凝乳蛋白酶抑制剂；研究了抑制剂在日本、中国和欧洲品种中的分布。F1杂种和亲本的电泳图谱比较表明，后代产生双亲类型的抑制剂。F2代和回交中的分离现象表明，在大多数情况下，每种抑制剂的表达由一对负责强带和无带的等位基因控制。真菌蛋白酶抑制剂C和D的两条带由共显性等位基因控制。这些结果表明，家蚕血淋巴蛋白酶抑制剂的多态性将是研究蛋白酶抑制剂遗传控制的一个有用的实验系统。
