12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯对人二倍体成纤维细胞表面糖胺聚糖的影响
Effect of 12-O-tetradecanoylphorbol-13-acetate on cell-surface glycosaminoglycans in human diploid fibroblasts.
作者信息
Wolff B R, Glick B R, Bols N C
出版信息
Can J Biochem Cell Biol. 1984 Dec;62(12):1354-7. doi: 10.1139/o84-173.
The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the loss of glycosaminoglycans (GAGs) from the pericellular compartment of human skin fibroblasts was studied. GAGs from this compartment were analyzed by anion-exchange chromatography after cultures had been labelled with both [3H]glucosamine and [35S]sulfate and then chased in either the presence or absence of TPA. In both control and TPA cultures radioactivity was found in glycopeptides, hyaluronic acid, heparan sulfate (HS), and chondroitin sulfate. The amount of radioactivity that was found in HS was reduced in the TPA cultures, whereas the amounts in the other GAGs were essentially unchanged when control and treated cultures were compared. These results suggest that TPA stimulates the loss of HS from the surface of human fibroblasts.
研究了12-O-十四烷酰佛波醇-13-乙酸酯（TPA）对人皮肤成纤维细胞胞周区室中糖胺聚糖（GAGs）丢失的影响。在用[3H]葡萄糖胺和[35S]硫酸盐标记培养物，然后在有或没有TPA的情况下进行追踪后，通过阴离子交换色谱法分析来自该区室的GAGs。在对照和TPA培养物中，在糖肽、透明质酸、硫酸乙酰肝素（HS）和硫酸软骨素中均发现有放射性。当比较对照培养物和处理过的培养物时，TPA培养物中HS中的放射性量减少，而其他GAGs中的量基本不变。这些结果表明，TPA刺激人成纤维细胞表面HS的丢失。
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