来自绿猴肝脏的溶酶体酸性脱氧核糖核酸酶——II. 酶学性质。
Lysosomal acid deoxyribonuclease from vervet monkey livers--II. Enzymic properties.
作者信息
Oosthuizen M M, Myburgh J A, Schabort J C
出版信息
Int J Biochem. 1984;16(12):1217-21. doi: 10.1016/0020-711x(84)90219-2.
Primate liver lysosomal acid DNase is an endonucleolytic enzyme. The enzyme has both 3'- and 5'-nucleotidohydrolase activities. The oligonucleotides produced by DNase are polymers mainly about 30 mononucleotides long. The Arrhenius plot shows a discontinuity with a transition temperature at 47 degrees C, with an activation energy of 107 kJ/mol below and 67 kJ/mol above this temperature. The activation enthalpy is 104 kJ/mol and the entropy -0.498 kJ/mol/K. The enzyme is subject to substrate inhibition and the Km value is 159 X 10(-3) mM DNA-P.
灵长类动物肝脏溶酶体酸性脱氧核糖核酸酶是一种核酸内切酶。该酶具有3'-和5'-核苷酸水解酶活性。脱氧核糖核酸酶产生的寡核苷酸主要是长度约为30个单核苷酸的聚合物。阿累尼乌斯曲线显示在47℃有一个转变温度的不连续点，低于该温度时活化能为107 kJ/mol，高于该温度时为67 kJ/mol。活化焓为104 kJ/mol，熵为-0.498 kJ/mol/K。该酶受到底物抑制，Km值为159×10(-3) mM DNA-P。
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