Littler J S, Scott C J
Biochem Soc Trans. 1984 Dec;12(6):991-3. doi: 10.1042/bst0120991.
A data collection system has been constructed, based on the low-cost BBC microcomputer, which provides for the digitization and storage of the data from one or more g.l.c. or h.p.l.c. instruments, or from other data sources with similar data rates. The data can be observed during collection on the graphics screen, and are then stored on disk for subsequent processing. This processing is designed to be interactive, so that the operator can influence decisions about base-line drifts, peak separations, etc. when integrating the peaks, and can decide which peaks are to be stored in a time/intensity record, on the basis of a visual display of the trace. A low cost multi-channel precision ADC, using isolated voltage-to-frequency transducers sited at the sources of data, and multiple counters at the computer, may be used to measure several signals simultaneously even when they originate at some distance from the computer, and extra memory can also be added to the BBC microcomputer to allow temporary storage of data. The software is written in machine code (for the data collection) and BASIC (for the analysis routines) so that modifications to the latter routines can be made easily. The user interface is suitable for routine users who have no computing experience.
基于低成本的BBC微型计算机构建了一个数据采集系统,该系统可对来自一台或多台气相色谱仪或高效液相色谱仪的数据,或来自具有相似数据速率的其他数据源的数据进行数字化和存储。数据在采集过程中可在图形屏幕上观察到,然后存储在磁盘上以供后续处理。这种处理设计为交互式的,以便操作员在积分峰时能够影响关于基线漂移、峰分离等的决策,并能根据色谱图的视觉显示决定哪些峰要存储在时间/强度记录中。一种低成本的多通道精密模数转换器,它使用位于数据源处的隔离式电压 - 频率传感器以及计算机上的多个计数器,即使数据源与计算机有一定距离,也可用于同时测量多个信号,并且还可向BBC微型计算机添加额外内存以临时存储数据。软件用机器代码(用于数据采集)和BASIC语言(用于分析程序)编写,以便能轻松修改后者的程序。用户界面适合没有计算机使用经验的常规用户。