Passechnik V I, Hianik T, Karagodin V P, Kagan V E
Gen Physiol Biophys. 1984 Dec;3(6):475-82.
Elasticity measurements of bilayer lipid membranes (BLM) based on registration of the third harmonic of the membrane current during the application of a periodic tension to the membrane was used to study the effects of lipid peroxidation (LPO) and phospholipase A on BLM. LPO resulted in decreased values of the Young modulus for BLM, while some products of LPO and phospholipid hydrolysis (linolenic acid) were able to increase drastically the modulus. The presence of individual products of LPO and phospholipid hydrolysis in BLM produced non-additive effects on the elasticity, strength and stability of BLM. Lysolecithine strongly affected both the strength and stability of BLM. without changing its elasticity modulus. It was found that the lower the rate of structural changes in lecithine BLM, the longer its lifetime. Membranes having a heterogeneous polar composition form more stable BLM as compared to chemically homogeneous membranes.
基于在对双层脂质膜（BLM）施加周期性张力期间记录膜电流的三次谐波来进行BLM的弹性测量，以研究脂质过氧化（LPO）和磷脂酶A对BLM的影响。LPO导致BLM的杨氏模量值降低，而LPO的一些产物和磷脂水解产物（亚麻酸）能够大幅提高模量。BLM中LPO和磷脂水解的个别产物的存在对BLM的弹性、强度和稳定性产生非加和效应。溶血卵磷脂强烈影响BLM的强度和稳定性，而不改变其弹性模量。发现卵磷脂BLM的结构变化速率越低，其寿命越长。与化学均匀的膜相比，具有异质极性组成的膜形成更稳定的BLM。
