胶原蛋白多肽模型的实验与理论构象研究。聚（甘氨酸-脯氨酸-异亮氨酸）和聚（甘氨酸-异亮氨酸-脯氨酸）

Experimental and theoretical conformational studies on polypeptide models of collagen. Poly(Gly-Pro-Ile) and poly(Gly-Ile-Pro).

作者信息

Tamburro A M, Guantieri V, Cabrol D, Broch H, Vasilescu D

出版信息

Int J Pept Protein Res. 1984 Dec;24(6):627-35. doi: 10.1111/j.1399-3011.1984.tb03169.x.

DOI:10.1111/j.1399-3011.1984.tb03169.x

Abstract

The synthetic polytripeptides poly(Gly-Pro-Ile) and poly(Gly-Ile-Pro) were studied both experimentally (mainly by circular dichroism spectroscopy) and theoretically by quantum mechanical methods. Poly(Gly-Ile-Pro) adopts a collagen-like structure under favourable conditions while the isomeric poly(Gly-Pro-Ile) does not. Theoretical studies emphasize severe intrastrand interactions which limit the main chain conformations in the case of poly(Gly-Ile-Pro). On the other hand, both the side cahin and the backbone in poly(Gly-Pro-Ile) can take up many different local conformations. Therefore, it seems that the conformational behaviour of synthetic polytripeptides can be at least partially explained in terms of local interactions.

摘要

对合成的聚三肽聚（甘氨酸 - 脯氨酸 - 异亮氨酸）和聚（甘氨酸 - 异亮氨酸 - 脯氨酸）进行了实验研究（主要通过圆二色光谱法），并用量子力学方法进行了理论研究。在有利条件下，聚（甘氨酸 - 异亮氨酸 - 脯氨酸）会形成类似胶原蛋白的结构，而异构体聚（甘氨酸 - 脯氨酸 - 异亮氨酸）则不会。理论研究强调了强烈的链内相互作用，这种相互作用在聚（甘氨酸 - 异亮氨酸 - 脯氨酸）的情况下限制了主链构象。另一方面，聚（甘氨酸 - 脯氨酸 - 异亮氨酸）中的侧链和主链都可以采取许多不同的局部构象。因此，合成聚三肽的构象行为似乎至少可以部分地用局部相互作用来解释。