在对核不均一核糖核蛋白颗粒进行微球菌核酸酶消化时，对核不均一核糖核酸的非信使序列进行优先核酸酶消化。
Preferential nuclease digestion of the non-messenger sequences of HnRNA upon micrococcal nuclease digestion of HnRNP particles.
作者信息
Pironcheva G L, Russev G
出版信息
Microbios. 1984;41(164):91-6.
PMID:6530960
Abstract
HnRNP particles from rat liver were digested with micrococcal nuclease to render about 50% of HnRNA acid soluble. Hybridization experiments, in which cDNA complementary to rat liver poly(A)+ mRNA was annealed to an excess of HnRNA, showed that the micrococcal nuclease resistant HnRNA fraction was enriched in mRNA-homologous sequences.
摘要
用微球菌核酸酶消化大鼠肝脏的核不均一核糖核蛋白颗粒，以使约50%的核不均一核糖核酸呈酸溶性。在杂交实验中，将与大鼠肝脏多聚腺苷酸加尾信使核糖核酸互补的互补脱氧核糖核酸与过量的核不均一核糖核酸退火，结果表明，对微球菌核酸酶有抗性的核不均一核糖核酸部分富含信使核糖核酸同源序列。
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