吗啡和（D-丙氨酸，甲硫氨酸）-脑啡肽酰胺对大鼠脑纹状体切片45钙摄取的影响。

Effects of morphine and (D-Ala, Met)-enkephalinamide on 45Ca-uptake to rat brain striatal slices.

作者信息

Saito K, Fujita N, Nakahiro M, Inoki R

出版信息

Neuropeptides. 1984 Dec;5(1-3):57-60. doi: 10.1016/0143-4179(84)90026-x.

Abstract

Effects of morphine and (D-Ala, Met)-enkephalinamide on 45Ca-uptake to rat striatal slices were examined. While morphine didn't give any effect on 45Ca-uptake to striatal slices in normal Krebs-Ringer solution, it inhibited K+ (40 mM)-stimulated 45Ca-uptake to slices. In high K+ medium, 45Ca-uptake was enhanced to 170% and this was reduced to 147 and 137% by 10(-5) and 10(-4) M morphine, respectively. Furthermore, the effect of morphine was antagonized by 10(-5) M naloxone. (D-Ala, Met)-enkephalinamide (10(-4) M) also inhibited K+-stimulated 45Ca-uptake to the slices to the same extent as was observed by morphine. K+-stimulated 45Ca-uptake to striatal slices obtained from chronically morphine administered rat (6 mg/kg, twice/day, 7 days) was not inhibited by morphine.

摘要

研究了吗啡和（D-丙氨酸，甲硫氨酸）-脑啡肽酰胺对大鼠纹状体切片45Ca摄取的影响。在正常的克雷布斯-林格溶液中，吗啡对纹状体切片的45Ca摄取没有任何影响，但它抑制了K+（40 mM）刺激的切片45Ca摄取。在高K+培养基中，45Ca摄取增强至170%，而10(-5)和10(-4) M吗啡分别将其降至147%和137%。此外，10(-5) M纳洛酮可拮抗吗啡的作用。（D-丙氨酸，甲硫氨酸）-脑啡肽酰胺（10(-4) M）也抑制K+刺激的切片45Ca摄取，其程度与吗啡观察到的相同。长期给予吗啡（6 mg/kg，每日两次，共7天）的大鼠获得的纹状体切片，K+刺激的45Ca摄取不受吗啡抑制。