Prokopczyk L, Jahn-Andrychowska W, Wańkowicz B, Kamińska E
Probl Med Wieku Rozwoj. 1984;13:198-206.
The in vitro studies on the metabolism of chloramphenicol in the liver, kidney and lung of 5 days old and adult rabbit as well as in the placenta (20-23 day of gestation) have been performed. The metabolites have been identified by thin-layer chromatography technique. Nonmetabolized chloramphenicol and reduction product were estimated by spectrophotometric method. The obtained results showed that in adult rabbit chloramphenicol was metabolized in the liver and lung, but no nitroreduction seems to occur in none of these tissues. In contrast to the adult animal the metabolism of chloramphenicol occurred in the all investigated organs of 5 days old rabbit. Each of the examined tissues of newborn animal showed the activity of nitroreductase. The biotransformation, including nitroreduction, of chloramphenicol occurred also in placenta. The thin-layer chromatography technique showed the presence of the product of hydrolysis of amide linkage in the liver of adult rabbit and in the placenta, and in the placenta additionally the product of hydrolysis of amide linkage without aliphatic amine group.
已对5日龄和成年兔子的肝脏、肾脏和肺以及胎盘（妊娠20 - 23天）中氯霉素的代谢进行了体外研究。代谢产物已通过薄层色谱技术鉴定。未代谢的氯霉素和还原产物通过分光光度法进行测定。所得结果表明，在成年兔子中，氯霉素在肝脏和肺中发生代谢，但在这些组织中似乎均未发生硝基还原。与成年动物不同，氯霉素在5日龄兔子的所有被研究器官中均发生代谢。新生动物的每个被检查组织都显示出硝基还原酶的活性。氯霉素的生物转化，包括硝基还原，也发生在胎盘中。薄层色谱技术显示，成年兔子肝脏和胎盘中存在酰胺键水解产物，并且在胎盘中还存在无脂肪族胺基的酰胺键水解产物。
