Galdal K S
Haemostasis. 1984;14(5):378-85. doi: 10.1159/000215095.
Endothelial cells (EC) in culture produce a procoagulant identified as thromboplastin when stimulated with 12-O-tetradecanoyl-phorbol-13-acetate, phytohemagglutinin, endotoxin, thrombin, histamine or epinephrine. Inducible thromboplastin generation most likely depends on de novo synthesis of RNA and the protein component of thromboplastin, apoprotein III. It is further probably regulated by intracellular mechanisms involving cAMP, transmethylation and calcium ions. The EC thromboplastin response is enhanced in lymphocyte-EC and platelet-EC cocultures and thromboplastin becomes partly available on the cell surface. Levels of procoagulant activity are reached which clearly may be of clinical importance if similar levels appear in vivo.
培养的内皮细胞（EC）在受到12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯、植物血凝素、内毒素、凝血酶、组胺或肾上腺素刺激时，会产生一种被鉴定为凝血活酶的促凝剂。诱导性凝血活酶的产生很可能依赖于RNA的从头合成以及凝血活酶的蛋白质成分脱辅基蛋白III。它进一步可能受涉及环磷酸腺苷（cAMP）、转甲基作用和钙离子的细胞内机制调控。在淋巴细胞 - EC和血小板 - EC共培养中，EC凝血活酶反应增强，且凝血活酶部分可在细胞表面获得。如果体内出现类似水平，那么达到的促凝活性水平显然可能具有临床重要性。
