Hill M A, Hobbs J B, Court J M
Horm Metab Res. 1984 Dec;16 Suppl 1:63-6. doi: 10.1055/s-2007-1014900.
A transparent tissue chamber adapted to the rat back was used for in vivo observation of vascular and circulatory changes in the streptozotocin diabetic rat. The chamber was implanted in a functional and richly vascularized bed of striated muscle. Extensive red cell rouleaux formation was observed within 2-7 days of induction of hyperglycaemia and was closely followed by development of constrictions and dilatations in both venules and arterioles. In 3 of 7 diabetic rats saccular microaneurysms, similar in form to those reported in diabetic animals and humans, developed in the vascular bed. The rat back chamber provides an opportunity for continuous in vivo study of vascular lesions developing in streptozotocin diabetes. Fixation under microscopic observation allows close correlation of functional changes in vivo with the ultrastructural appearance of specific tissue areas.
一种适用于大鼠背部的透明组织腔室被用于对链脲佐菌素诱导的糖尿病大鼠的血管和循环变化进行体内观察。该腔室被植入有功能且血管丰富的横纹肌床中。在高血糖诱导后的2 - 7天内观察到广泛的红细胞缗钱状形成，随后小静脉和小动脉均出现收缩和扩张。在7只糖尿病大鼠中的3只，在血管床中出现了囊状微动脉瘤，其形态与在糖尿病动物和人类中报道的相似。大鼠背部腔室为连续体内研究链脲佐菌素糖尿病中发生的血管病变提供了机会。在显微镜观察下固定可使体内功能变化与特定组织区域的超微结构外观密切相关。
