Honda H, Kodama R, Takeuchi T, Yamanaka H, Watanabe K, Eguchi G
J Embryol Exp Morphol. 1984 Nov;83 Suppl:313-27.
Cell monolayers on culture dishes were divided into two groups: tensile monolayers and non-tensile ones. In the development of an epithelium, a non-tensile cell monolayer turns into a tightly bound tensile one. Detection of these states was carried out by using the boundary shortening procedure, a computer-based geometrical method to show how much the polygonal cell boundary contracts. Non-tensile monolayers were divided further into two groups according to their motility: a fluctuating monolayer in which cells move laterally, and a stable monolayer in which cells are immobilized. Quantitative determination of cell motility was performed by analysing time-lapse cellular patterns. These computer-based geometrical analyses enabled us to divide monolayers into three groups: tensile stable monolayers, non-tensile stable monolayers and fluctuating monolayers, and this study therefore gives an insight into the way in which changing conformations of cells may be assayed.
拉伸单层和非拉伸单层。在上皮组织的发育过程中，非拉伸细胞单层会转变为紧密结合的拉伸单层。通过使用边界缩短程序来检测这些状态，这是一种基于计算机的几何方法，用于显示多边形细胞边界收缩的程度。非拉伸单层根据其运动性进一步分为两组：细胞横向移动的波动单层和细胞固定的稳定单层。通过分析延时细胞模式对细胞运动性进行定量测定。这些基于计算机的几何分析使我们能够将单层分为三组：拉伸稳定单层、非拉伸稳定单层和波动单层，因此这项研究深入了解了检测细胞构象变化的方式。
