鸡视网膜中视杆色素与四种视锥色素的色谱分离。

Chromatographic resolution of the rod pigment from the four cone pigments of the chicken retina.

作者信息

Yen L, Fager R S

出版信息

Vision Res. 1984;24(11):1555-62. doi: 10.1016/s0042-6989(84)80005-x.

Abstract

Chicken rod and cone pigments are chromatographically resolved using a combination of stepwise elution from a concanavalin A affinity column and a secondary ion exchange purification. The rod pigment rhodopsin and the cone pigment iodopsin are purified to homogeneity. There is a partial separation of three other cone pigments: chicken green, chicken blue, and chicken violet, making clear that this is at least a tetrachromatic cone system.

摘要

利用伴刀豆球蛋白A亲和柱的分步洗脱和二次离子交换纯化相结合的方法，对鸡的视杆和视锥色素进行色谱分离。视杆色素视紫红质和视锥色素视紫蓝质被纯化至同质。另外三种视锥色素（鸡绿色素、鸡蓝色素和鸡紫色素）得到了部分分离，这表明这至少是一个四色视锥系统。